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- Flowjo 10 0 5 Crack (flowjo-10-0-5-crack.torrent.rar.zip) in free image graphics lake can download by Megaupload Rapidshare 4share Torrents uploaded.net Emule. The main analytical focus of the most widely used industry software, such as FlowJo and FCS Express, is manual gating.
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Download FlowJo 10.0 for Mac free latest version offline setup. FlowJo 10.0 for Mac is a professional application to analyze and display the flow cytometric data and provides a variety of analysis tools for microscopic particles.
FlowJo 10.0 for Mac Review
The FlowJo 10 is among the best analysis applications for handling the FCM data i.e. Flow Cytometry. The application provides complete support to display and analyze the cytometric data as well as provides complete support for analyzing the microscopic particles i.e. cells and chromosomes etc.
The flow cytometer extracts the properties of the cells and the phenotypes using the fluorescence emission. After collecting the data, this powerful application analyzes and displays the flow data. It is also possible to display the data in single or multiple dimensional histograms.
It makes very convenient for the researchers to study any health disorder and make it possible to check for any cancer cells. Change various properties and extract useful information for both clinical and research purposes. All in all, it is a reliable environment for analyzing microscopic particles and provides complete support for Flow Cytometric data.
Features of FlowJo 10.0 for Mac
Winning eleven 8 pc completo. Some of the features of FlowJo 10.0 for Mac are:
- Powerful analysis application
- Analyze the flow cytometric data
- Uses Flow Cytometry or FCM
- Count and analyze the flow cytometric data
- Analyze the microscopic particles
- Displaying the flow data as 2D and 3D histograms
- Logarithmic measurements with higher precision
- Helps in studying the health disorders and cancer cells
- Define different instances in multiple groups
- Change the properties of the groups
- Perform different operations individually
- Many other powerful options and features
Technical Details of FlowJo 10.0 for Mac
- File Name: Tree_Star_FlowJo_X_10.0.7_R2_macOS.zip
- File Size: 72 MB
- Developer: FlowJo LLC
System Requirements for FlowJo 10.0 for Mac
- Mac OS X 10.6 or later
- 2 GB minimum RAM
- 1 GB free HDD
- Intel Multi-Core Processor
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Flowjo 10 0 2
For a digest of these release notes, please see this page. This page contains an exhaustive list of issues that have been reported and fixed, new features implemented, and known issues.
Fixed:
Flowjo 10 Crack
FlowJo 10.0.7r2:
- Significant Improvements:
- Compensation is now “remembered” in templates
- Compensation population size restriction has been reduced to a warning if under 5%.
- Manual gating will override matrix calculation and allow matrix to calculate
- Cytek data transforms fixed
- Miltenyi fcs3.1 concatenate files scale issue
- 10.0.6 workspace opened in 10.0.7 could drop parameters
- Stability,and other issues addressed:
- Some annotation fields did not allow text entry
- Comp Matrix Editor could stay open open with an applied Matrix when closing workspace.
- Export FCS now includes changed keywords. (It has not in the past.)
- Two bisector gates on population produced a 2D gate
- Table Editor: Fixed Keywords menu to not filter based on what was already used.
- Heat Mapping Without Values now works
- 10.0.6 workspace data reconnection issue resolved
- (rare) v1006 workspace wont open in v1007 – fixed
- FCS2 Portion of Galios Data was not always visualizing correctly
- Several cosmetic fixed including dialogs and tip text rollovers have been improved.
- Previous Arrow icon in Preferences is backwards.
- Gate Naming Dialogue – restored autocomplete from favorites when entering gate name
- Contact FJ form did not not appear in all cases.
- Text in new gate naming dialogue is not highlighted
- Binding Node Icon created
- Linux Version:
- linux graph data scaling issue fixed
- IAE in launcherDialog when exiting in Linux in VMware
- Several broken links to docs were fixed
- BMS Version
- BMS version – Table exports overwrite each other
- BMS version – The Select button for setting the output path in prefs is cutoff
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- Annotation
- Clicking Annotate Experiment in Plate Editor throws a stack.
- Annotate Exp. Figures Tab: Divider between the Figure table and the preview acts strangely
- Annotate Experiment Instrument Panel Panel & Comp. text fields don’t get along
- Boolean Gates
- Boolean tools all throw stacks and do not create valid gates
- Creating Combo gates throws a stack.
- Creating NOT gate on grandchild causes the stat of the gates to be lost
- Cell Cycle
- FJ is still calculating after CC is applied.
- Cell cycle throws stack at open
- CC File:Print… Menu throws a stack
- CC1D platform arrows not working (throws quiet stacks)
- delete cc Node = stack
- Switching bivariate samples using arrow buttons can throw stack
- switching rapidly in bivariate sometimes show quiet stack
- CC throws stack on FACSArray file with no S Phase Marker
- CC tutorial data is not being scaled correctly in the CC platform
- Cell cycle Multivariate gates can’t be dragged
- Cell Cycle Go Menu has blank entry as first menu item
- Cell Cycle Bivariate Y Axis has no label
- Cell Cycle Bivariate gate labels all piled up at top left of chart and non selectable or moveable
- Cell Cycle Bivariate T buttons do nothing
- Cell Cycle Bivariate right click menus are a mess
- CC1D gate disappears when dragged
- CC File:Save Graphic As… menu looks like it works but does not save
- CC tooltip for Calculate… menu undefined.
- CC Edie Menu options do not seem valid for CC platform?
- CC Help Menu all have bad links…
- Cell Cycle on non cell cycle data show warning but still opens platform
- Copying CC node to group and switching from univariate to bivariate and back throws stack
- CC1D mean peak stat incorrect
- CC1D stats in TE are all “0”
- TE formula references are all “Cell Cycle/Freq. of Parent”
- Deleting CC node from Group doesn’t eliminate from WS
- CC1D G2 and S phase stats overlapping on GUI
- CC Multivariate EM Has extra widgets
- Stringnames are shown for Copy Stats and Preferences in Edit menu.
- CC 1D > G2 shows 0% in Platform
- CC Next and Prev. arrows only partially update platform window
- CC creating CC on more than one sample throws stack
- 1D CC Re-open workspace with CC, CC node opens blank CC window
- CC Group owned CC opens empty window and does not generate statistics
- CC gate name and freq. of a Bivariate plot are piled up
- CC chart stripped of analyses when applying save graphic as
- CC in Simple Mode Bivariate & Multivariate stacks and no gates created
- CC Changing the Bivariate gates does not trigger a recalculation of the Multivariate gates
- CC Dragging CC node to LE makes not a pretty picture
- CC Drag Dropped CC Node to LE = Stack & no image
- CC First create of CC Note, Bivariate gates are bad
- next / prev don’t update Y axis
- Opening multiple CC platforms, the windows don’t offset like other platforms
- cell cycle stat -100
- Add 1d stats to workspace
- label Bivariate & 1d stats in workspace
- Duplicate 1D stats appear when arrowing through CC samples
- Unable to analyze multiparameter CC data file, cutoff warning message, no parameter selection
- All stats are zero for LMD Cell cycle data
- Cell Cycle Stats mean peak stat incorrect
- CC & LE… graph in LE changes with Platform
- CC in LE changes graph type depending on Platform window
- Cell Cycle Multivariate EM right click menus non fucntional
- Cannot get past biomarker selection interface in CC test set 5
- loading a saved workspace with cell cycle analyses shows -100 stat
- CC 1D Drag into LE repositions S phase marker
- CC1D histogram scaled differently in CC node vs LE after Transform.
- CC1D G1, G2 (peak), S-phase stats incorrect
- CC1D stats in TE incorrect, do not correspond to GW
- Dragging CC node with different view in CC sets shows overlays in LE.
- Setting region constraints
- CC Table duplicate labels, first one should be < 2N
- Re-Opened workspace does not show 2D & EM CC Data
- CC 1D Change X parameter not permitted
- CC 1D constrain ratio yields strange 4N stats.
- Unknown stat on CC 1D GUI
- Statistics added to cell cycle nodes disappear upon constraint application
- CC platform is not saving its constraint state upon application
- CC1D GUI stats incorrect (Update issue)
- CC1D constraints checkbox drops WS stats
- CC1D node gates incorrect
- Reopened workspace BiVariate CC showing wrong Y param
- CC 1D Constraining CV and Ratio drops CV constraint
- CC Re-Open Workspace, null param warning (better STRs)
- able to create zero width constraint gate with strange effects
- CC & LE… graph in LE changes with Platform II – New STRs
- CC 1D does not remember what axis param was chosen
- CC Biomarker Selector Dialog Cancel does not cancel.
- CC1D TE cv stats incorrect
- CC1D 2N/4N gate snaps to same location in vertical
- Double-click CC node changes X-axis parameter
- Command Line: commanjo needs tooltips
- Comp Matrix Editor
- Clicking Edit Compensation Matrix button is throwing a stack
- Overlay Uncompensated button in the ME is not previewing the samples and throwing a stack.
- Overlay Uncompensated on Comp Ctrls is not on by default though checked and throws stack when unchecked.
- There is 2 hidden unselectable options in the ME.
- Stack when clicking the View Matrix button in Compensation and graphs are missing.
- Matrix looks distorted when opened through the badge of a sample
- compensation editor problem reported by helene
- ME doesn’t fill background to edge, and scroll bar thinks height is larger than it should.
- loading multiple compensation matrices from files
- Compensation Calculation with 13 color data set provides no user feedback to correct gates
- Formatting for Positive Negative control gates in overlay does not match GUI coloring
- Updates to positive gate not reflected in overlays in CME
- Unable to select the color palette.
- Compensation
- Compensation Dialog does not open and throws a stack.
- MoFlo Data Doesn’t Show Any Parameters in the CE
- NPE in cytek data handling
- Comp Ctrls, Sample, Neg and Pos vibrate when mousing over them. PC Only
- Right click menu on badge to add comp shows string name
- Stain name is lost and Parameter name highlighting not present in a reopened WSP
- Compensation Window Tooltips
- Comp wizard drop-down doesn’t add population.
- Compensation dialog menu stringname tooltip issues.
- CE <Reset This Parameter> clears instead of resets it
- Compensation Menus on Positive and Negative samples need to be cleaned up.
- Derived Parameters
- derived parameter window changes size when option panel selected
- Increasing Derived parameters minimum range is throwing a stack
- Can’t create Derived Param
- Event# parameter not available for derived parameters
- derived parameter node renames itself
- Custom transform on Derived Param is not restored from workspace or Template
- Transforms are not being applied with Derived Parameter when group applied.
- Dialogs
- Save & Save As will overwrite existing file with no warning
- Changing the Sorting order is removing data in find/replace
- Show all keywords in menus checkbox in modify group dialog not staying selected after applying
- Stringname is shown in the Group dialog for the Any Keyword selection.
- “You” tab of Startup License Sequence shows extraneous “Next” button
- Open, save and non specific save as dialogs are not using ideal osx dialogs
- Adding non demo file to demo mode brings up wrong dialog
- AutoQuad tool tip showing string name
- Create Cell Cycle Gates tool tip showing string name
- Annotation dialog / Plate & Experiment text fields need to resize
- Group Dialog tooltips largely undefined
- Manual gate dialog has display issues
- Next button should to be removed from the License tab.
- The Date fields in the Annotate Experiment dialog is not fully shown.
- no error message when os is switched to offline mode while running FJ shipping version
- unable to add stat thru stat dialog
- sentence cut off in proxy settings dialog
- Don’t Show Again does not work with Rename for all on Group owned gate.
- Don’t Ask…Delete group stops asking and stops deleting,
- Are you sure you want to create and open X platform windows dialog controls are reversed
- Keyword Value Series… Dialog does start with correct number of edit boxes
- Manually Enter Gate Instructions are Editable
- Keyword Popup Does not protect from going off the bottom of the screen (Mac only)
- Stringname is shown for the Tooltip for Dont Show Again checkbox
- DIVA FCS2 warning has extra buttons
- New in VX Link in About Dialog does nothgin
- Can’t cancel ACS open from Workspace Version dialog
- Document – WSP
- Transform of a Derived parameter is not saved in the workspace
- Workspace with no Samples looses created layouts
- Export
- save as excel from the workspace throws NPE
- data export issue reported by Chris Bray of Verity Software House
- exporting from the workspace is causing FlowJo to crash
- Group export of Sample Data locks up with Engine 406 Error
- These Coulter Files don’t Concatenate Properly
- Exporting CSV does not save any additions made.
- Concatenate in Export dialog shows stringname for tooltip.
- FCS Scan
- FCS Scan Stacks when button pushed
- FCS Scan fails to list FCS files from “Documents” folder
- Help button is replaced by a text when Show Duplicates is checked.
- File Formats
- Miltenyi Data in MQD format not displayed correctly
- Stack opening Template (pre-fix) with Layouts
- vX cannot open FlowJo 9.7.1 Jo files
- Re-open workspace Error:target population does not have the Comp…” on re-opening the workspace.
- 9.7.1.jo file drag to open jo file does not create a new workspace
- Graph Window
- Opening Parent in graph window throws stack… specific case only!
- Delete Gate with child open throws 2 stacks
- NPE When Opening a group gated population on comp
- Graph Window Make Movie Stack
- clicking y or x axis parameter menu in graph window is throwing a stack
- Can’t create a range gate dragging left to right
- Not loading in any data from this LSRII Data Set
- Fast Preview no longer appearing
- IsFloC: Custom Transform Graph bad when linear and min is close to 0 (specific sample Gain Related?)
- Graph Window Up Arrow no longer shows parent population params with the gate
- Spider gate Q1 is showing X/Y coordinates in GW – W7 only
- manual gate – cannot mix percentage & intensity
- Gridlines do not display on LE
- Curly quads cut off upon resize
- Gate Annotation on Graph window Snaps to right edge from too far away (Very strong magnet!)
- Graph window Increase & Decrease Gate Size buttons don’t work on Rectangle Gates
- Decrease size button on Graph Window does not visually show when available
- children plots not updating when parent gate is moved
- manual gating dialog seems to be missing action buttons
- can open multiple graph windows
- Control gates do not keep their value when applied to a sample
- Continuously moving any of the Quads slows down FJ.
- Spider gate turns into a regular quad when adjusted inside another type of gate.
- Quads 1 & 3 change annotation on transposed axes
- Transposed curly quad gates move operation incorrect
- Spider gate creates an extra line when changed from Lin to Log or Biex.
- Q1 annotation on a Transposed Spider Quad is cutoff
- Can’t delete quad gates in Graph window
- Opening the Child of any of the 3 Quads through the GW will view the Parent GW.
- Deleting a Quad from the GW removes Comp Ctrls and the Badge of the sample is blank.
- close and reopen GW resets gate annotations
- FlowJo not displaying time parameter
- biexp scaling on histos Y axes
- Offset quad gates showing duplicate edges
- spider gate legs don’t show handles
- Off-axis gates snap back on Miltenyi FCS3.1 Data
- Curly quads shift location in GW
- Cytof Events on Y axis don’t show up in the gate on the edge
- Remove sound for GW opening.
- Positive Annotation to be shown on a 2d plot after a bisector is created on a histo.
- right click menu showing string name
- GW Open Child Population should be disabled with no gate selected
- Can’t drag annotation on 1D chart
- In Simple Mode, some Graph menus are missing all items
- Gates can not be dragged to top of graph
- Giant Curly Quad Gate from template and does not draw in graph
- Spider gate with transposed axis bad things happen when moving end points
- Transpose axis with Quad or curly quad the annotations & stats don’t update
- Transpose Axis with Quad shows a box around Q1
- Copy Histogram to excel copies incorrect population
- Bisector gates open with unfilled histograms (when pref is set to filled histos)
- Miltenyi Transform tooltip shows stringname.
- Quad gates are not drawing vertical bar when asymmetrical
- Range gate won’t deselect if in same graph as Bisector Gate
- Bisector gate’s population opens in psudocolor instead of Histo.
- Stain name parameter requires click-refresh in WS before application to GW
- ec800 X and Y scales are not being read?
- Graph window of child opens stretched if any options panel is open in the parent
- FJDotPlot indexing inconsistency
- Quad gates are deleted without confirmation dialog
- Breadcrumb trail in graph win goes bad when adjusting cc gates
- Annotation in the GW can be cutoff only on the rightside.
- Editing Range gates in 2D plots doesn’t constrain movement
- Graphics
- Exit Icon needs revision
- Export icon improvement needed
- Experiment needs new icon
- Populations icon needs work
- Edit comp matrix icon work…
- Check Sample Quality icon still needs check mark moved left to be clearer.
- Keywords icon needs work
- We need a Chart Properties icon
- Properties. icon needs changes
- Launcher window needs re-design
- Plate Preference Panel needs icon
- Panel Wizard icon in Prefs needs improvement.
- Preference dialog Previous and Next buttons should have arrows and not dots.
- Icons for disclosable panels
- PE Icon changes…read from workspace, move wells to workspace
- File Save Icon using wrong artwork
- Icon for plate “deselect all” action
- Transform Axis Window is using old arrow art for next & prev arrows
- derive parameters Icon could use a revision
- Grids
- Stack is thrown in the LE when making an overlay in a grid
- Ability to open Graph Def and edit a graph that is in a grid
- Too easy to move charts out of a grid
- Shadow changes the number of cells seen in a grid
- Right click menu functions on Grids need tooltips.
- Tooltip shows stringname for Enable Iteration and Scale Figs on Cell Resize.
- Kinetics
- Smoothing options do not work
- most settings do not change graph
- Closed options panel show missing string(s)
- Launch: Opening a saved WSP by double clicking on it opens two and one is blank
- Layout Cell Cycle
- Y Axis of CC changes when added to the LE.
- Cell cycle batched label goes blinky
- Layout Charts
- Cannot change graph type in LE
- Stack is thrown when selecting All Parameters by Y from the right click menu in the LE.
- Dropping a Stats Table LE tool into a Grid throws a stack.
- All Layout tools overlap the ruler when a Histogram Stagger Offset plot is selected.
- Adding columns to legends does not work on multiple plots
- Ability to change x/y annotation on multiple plots in the LE
- Ancestry is still shown after unchecked in the Graph Definition.
- Batched Table does not reflect the Fill and Gradient until it is clicked on.
- Out of Memory adjusting Zoom on a Batched NxN plot.
- Disabling the Enable Iteration does not work on Grids.
- Backgating is still shown after unchecked in the Graph Definition.
- bg/fg colors not recognized in layout editor charts
- “no line” setting on a graph in the layout still shows a faint line
- adjunct histograms overextend selection handles when batched
- Change the wording for Remove Cells From Grid.
- LE graph def parameter list error
- runaway x axis tick
- All figs, FG and BG color lost after apply and OK
- grid gets painted past chart in the LE
- Colors in overlay legend are not showing
- Ancestry & Backgating on Overlay should not be options
- Ancestry/Backgating shows label far to the right of the chart for Bisector gates
- Bisector Gate in Graph in LE missing 2nd Stat Label
- Mismatched Iteration warning in LE not appearing for Overlays
- Draw Rectangle Gate in 1D graph
- wide data crashes NxN
- Layout Editor
- Plate Tool on LE – gone when re-opening the WSP
- Batching with statistics in the LE is throwing errors
- Re-Open Workspace Layout Editor has phantom layout
- Curly Backgating doesn’t work
- Lock Content in the LE does not work on Text ribbon functions.
- Graph Definition changes only affect one of multiple selected samples.
- LE Text Box Insert Keyword Menu malformed
- Cannot rename the X and Y axis labels through the Graph Definition.
- Plate Tool on LE rollover text is incorrect
- LE Properties Panel has inverted icons for show & hide
- sporadic CME in complex layouts rebinding samples
- LEBatch Band : The Across vs Down radio group always reverts to Down after creating the batch.
- Stringnames are shown for the Tooltips under the Annotation tab in the Graph Definition
- LE after dragging a Guide line, the cursor does not change when hovering over the guide until the selection changes
- Can’t grab the page break control in the LE if a fig is on top of it.
- Move Figure Presets from Ribbon to Properties:Info in LE
- easy way to NPE in LECHartController
- layout editor window resizes when closed an reopened
- Graph Definition window not adding/deleting populations
- unable to paste image into le
- save figure as in the le is not working
- Batch to pdf in layout
- Clicking the Add New Layout button in the LE does not select the New Layout.
- Bisector Histogram annotations not added to LE
- LE Batched Table: Set Cell Padding Does not grow table to min size until you try to resize it
- LE Grids: Remove X and Remove Y Axis menus incorrectly enabled
- LE Dragging object to combine them work only under certain conditions based on creation order
- Multigraph Overlay issue with 2D graphs
- Tooltip shows stringname for Edit in right click menu.
- Histogram Overlay Y Axis Modes Not Applying
- Statistics Dragged Into LE Not Displaying Properly
- Layout’s line tool keeps line horizontal during creation
- Line tool resize moves other point
- LE Paste should past into the visible part of the window
- Applying Fig preset does not update the Properties Band
- LE Grid Right Click Menu using wrong popup
- Iterating Layouts with Page Up/Dn doesn’t refresh Iteration band
- pagination not stored in layouts
- If the copied figs are too close to the edge of the LE, the paste piles them up in the corner
- CDF scale changes in LE
- Group Boundaries in LE Too Large
- Icons missing in the LE & PE Lists
- Multiple instances of the Layout band fails in the LE
- No scrollbar after undo in LE
- Layout Iteration
- Iterate by Sample selection in LE is throwing a stack with CC data loaded
- Stack is thrown upon Panel Iteration selection
- Multigraph All Params causes bad layout with comp populations
- workspace and sample iteration naming conflict
- can’t deselect after batching
- Iteration Off contains Value drop down list when Batch to Printer is cancelled.
- Iteration Values are reset to default setting after batching.
- Layout Outputs
- Save Button & Cmd S throw NPE Stack
- Stack is thrown when overwriting an opened batched PDF from the LE
- Ability for graphs/object to have transparency when outputted to PPT
- Batching to web page shows density plot very pixalated
- Show Outliers function doesnt show when Batched to PDF
- Batched Layout no longer saving the isBatch flag in xml file
- append to powerpoint fails when batching
- Create Batch never finishes if specified path no longer exists
- <DocumentName/> in Header or Footer not available when printing
- Disable append to PowerPoint for now
- ‘Save Image As. pdf From LE is not including headers/footers in produced report
- Layout Overlays
- creating an overlay causes the rainbow wheel of death
- Plot retains wrong color in LE Remove Layer
- Line style should show in the legend
- Legend is blank on an Overlay.
- Staggered Histograms only display transform lines for linear params
- change all layers of the histogram overlay at once
- Legend color box doesn’t fill on Histogram Overlays
- Layout Plates
- Selecting Cut on a Plate Tool throws a stack.
- Dragging Plate Tool throws a stack.
- Stack adding plate data to template with Plate in LE
- Plate tool in LE is not populated when loading template
- LE save image as… plate tool cuts off annotation and left labels
- Rich tool tip for LE Plate Tool has incorrect description
- NPE When deleting a non group owned gate if a Plate is open in the LE
- Cant edit well id (was: Plate Tool missing “D3” well info)
- LE Plate tool is using Plate Editor Prefs and it should not be.
- Layout Plates Missing Annotation
- Links
- Web doc naming scheme out of synch with FJ’s
- Help button on Window when no workspaces are open leads to 404
- kinetics help web link broken
- proliferation help web link broken
- redundant links in ws help band
- MIFlowCyt help web links not working
- keyword value series help web link
- iterate by keyword dialog help web link broken
- web link in le iterate by keyword diag broken
- no help web links in the pe
- PE All help links= 404 Errors
- Kinetics Define Threshold Value dialog help 404 error
- Other
- Keyword Popup with search no longer searching
- FlowJo window has transparency issues.
- FlowJo Finder version is 1.0 on osX
- Popups do not dismiss when FlowJo loses focus.
- Panel Wizard: Opening the panel wizard in offline mode is throwing a stack
- Plate Editor
- Plate editor & LE Plate Tool is not using Pref for Well ID
- Stack when opening Plate Editor with no data
- Clicking Cancel for Set Plate Dimensions throws a stack.
- Clicking Read From Workspace throws a stack when data is in the WS.
- PE: Select Group Stack
- Clicking the Add Keyword/Value button throws a stack.
- Opening workspace > 96 well plate, shows stack when trying to open PE
- Stack is thrown when importing BAD CSV into PE or through drag and drop.
- importing a csv is causing a stack
- Plate editor import throws stacks on import
- PE Wells should not be allowed to contain multiple instances of the same keyword
- PE Remove Staging Well does nothing
- PE Open… Does not open a files saved with Save
- Plate Meta Fields are not cleared(or restored) with multiple plates
- PE Paste is not pasting into the selected cell
- PE Filter Drop Down is hard coded and should be dynamic from data
- PE – Well display does not update when using arrow keys to move selected well
- PE Keyword Filter changes
- PE Changes to Staging Wells
- PE Well Display Area changes
- PE Modify name and function of Read from Workspace
- PE Move Wells to Workspace needs to change name and function
- PE Use the Circle drawn on a well to indicate an associated file in the workspace
- PE: Heat maps showing only BLACK Wells
- Plates show contents of some wells and not others
- staging well right click duplicate not duplicating
- Copy and past in PE changed WELL IDs
- Plate Editor, when wsp reopened loses plate name, attributes and user annotations…
- Plate Tool title is “…Under Construction…”
- In Plate Editor, new plated do not have a default name
- Plate Editor “Add” button does not look like a button
- Plate Editor Pencil button has bad rollover text
- Plate Editor continuous spew of data to the console
- PE Plates do not close when the associated workspace closes
- reopened workspace Saved Plate is missing data and strange
- PE: Selecting different plate in plate list does not update the well information panel
- Ignore duplicate keywords in drags to wells
- Plate Editor needs WindowPosition persistance
- Plate name edits in sidebar revert when switching plates away and back
- Plate editor staging well drag throws stack
- Make Group makes a group with only one file in it despite well selection
- Opening a saved plate XML file in plate editor
- PE Annotate Experiment Dialog opens too small
- Plate Editor Read from WS not populating all wells
- set plate dimensions
- bug uncovered at high plate size
- Not everytime will a keyword drag and drop to a cell and Staging Well in PE. PC Only
- PE: Plate is sized wrong row & col count reversed
- Set Plate Dimensions does not work.
- PE Make Group Help Button no-op
- PE Cut, Copy Paste not working
- PE Creating keywords automatically adds the keyword to the selected cell
- PE: Show Attributes button highlight is reversed
- PE Clear Selected Wells does not clear wells loaded from workspace
- PE Show heat maps button is highlighted reversed
- PE Read from workspace multiple times duplicates keywords
- PE Bottom Panel (staging wells etc) is too easy to separate from the Plate window when resizing the plate window
- PE add Clear Selection button
- PE Import Tool tip needs additional information
- PE: Color Borders of categorical keys button does nothing
- PE Use Thicker Borders does nothing (as far as I can tell)
- PE:Don’t allow user to add empty keyword/value pairs
- Rename staging well, dialog should default to current name selected
- Selecting a named group does not clear selected staging well
- PE: no way to remove a named selection of wells
- Dragging Staging wells onto another staging well
- PE should not use samples from workspace until user tells it to
- Editing Fields at top of plate cuts off font
- PE Keyword field should not be editable. just the value field
- Remove the ADD button from the PE Keywords Band
- PE: Wells should not associate with samples until the user chooses to do so
- Plate Editor not using Well ID Plate Pref for Import csv file
- Apply Plate Keywords to Group not working.
- Keyboard commands for cut, copy & paste don’t work with plate editor wells
- PE disable Make Group button if wells are not associated with samples
- Change value of a keyword in a well should ONLY change the selected well
- Copy multiple Wells and Paste in PE = Stack
- Remove staging well button still active even when no staging wells are selected
- New plate in PE needs new color and default name “-copy”
- PE Dragging lots of Keywords to a Staging Well breaks the UI
- PE The horizontal layout of the draggable bottom panel looks terrible in vertical mode
- PE Saving a workspace with a plate, upon re-opening, always has an extra plate in the list
- Dragging staging well to well, row or column shows stack
- Plate Editor Set Plate Dimensions… dialog need beautification
- Plots
- Time series values ordered by workspace sort, not Time
- Time Plot of 5 Stats = Stack
- Histogram plots in the LE should keep the default color to match the GW plot.
- Y axis label in time plots should adjust when window is rescaled like the x axis
- Population Comparison: Population Comparison Batching in TE problems
- Preferences
- stack thrown at keyword/value pair deletion
- Adding Comp Pref Keyword that does not exist in fcs = STACKS
- Stacks when opening overlay settings menu
- the panel wizard icon coloring is inconsistent in prefs window
- Default Plate Size pref is not affected by the Reset button.
- Plate preferences switched
- CC Prefs – General
- Workspace Sort Order Pref not in effect for future workspaces NEW STRs
- Incorrect Sentence Checkbox Tip
- fav gate names session-transient (not stored in prefs)
- Reset Prefs should not reset License state
- default value change in Number of Local Engines pref
- Splash Graphic Preference Not Persisting
- Prefs dialog needs to be made modal to the Application
- Header & Footer Table Prefs not working
- TE Pref remove the Close Tables without saving checkbox
- Layouts Pref ‘Fill Histogram Overlays’ Not Working
- Annotation Prefs- Can check ‘backgating’ when ‘ancestry’ isn’t checked
- Annotation Prefs – Backgating Not Working
- Missing string but it’s in the dictionaries
- Plate ID in Plate prefs is showing a stringname.
- Entering decimal numbers with Country language pref set to Germany
- Compensation Prefs Remove Button no-op
- compensation preferences text is cutoff
- Matrix Editor section shifts down and disappears from clicking the Add button.
- Editing Comp Prefs Keywords adds bad criteria to comp group
- Plates Prefs – Intended Feature Doesn’t Exist
- Unable to choose what to delete. Each row should have a delete
- Layouts Prefs – Panel is cut off
- Printing
- Cannot scale Table to one page for printing
- Footer Treestar Icon disspaears when changing between Paper styles in Landscape Orientation – pc only
- Padding in Print dialog needs a limit.
- Print preview through the GW splits a single sample into 2 previews.
- Printing a histo plot from graph window is missing the color fill.
- Global Print dialog – Reset does not affect Rows and Columns digits.
- layout page breaks are not used in the print dialog
- Header/Footer Treestar info is missing in Print.
- Avoid page breaks moves graphs all over the place
- Refresh Issues
- Y axis scaling changes when graph defs are accessed – refresh issue
- Heatmapping doesn’t apply to stats table – refresh issue
- Use placeholders is not working in the LE
- Ribbons
- Dragging off a ribbon on collapsed tab mode throws a stack.
- file tab; document ribbon save as… should be Save As Workspace…(
- Save As Workspace… Brings up dialog looking for ws.save.workspace
- context “freq. of” actions from the Statistics band in Workspace tab – no worky
- Ribbon sub-menu’s extend out of visible range
- Figure Presets ribbon only works in the farthest right tab when used in multiple tabs.
- Figure Presets Apply & Plus button should be disabled if there is no figure selected
- Ribbon bands don’t go away when popped up
- Removing a collapsed Biology band closes FJ but is still running. PC only
- Sample Quality: Sample Quality Check throws stack if a group is deleted.
- Script Editor: script editor throwing stack upon running script
- Shortcut Keys
- The Enter key becomes the Undo shortcut key on a Curly and Spider Quad.
- By default the FJ icon and the FlowJo tab have the same ALT shortcut.
- Statistics:
- Statistics Ribbon- Validation Stack when adding Median or CV from icons
- Dragging stat to the group, dups stat and contains n/a copy.
- Frequency of Statistic for Booleans not copying correctly to Workspace.
- When adding a ‘Freq of’ stat, the name is repeated twice
- Adding freq. of stats from stat interface
- Stats not calculating in workspace
- Percentile Statistic dragged to group does not work
- Table Editor
- Stack is thrown refreshing from the WS with a Batched Table in the LE
- Error appears and a Stack is thrown when Batching to Printer
- Heatmap tables does not output to the LE
- Additional info on Y-axis in formula time series graph
- Table Editor needs a scroll bar for stats
- table column sorting error
- batch to table, sort table, double-click – GW has wrong sample
- Remove Column header name or show full name.
- Table Editor selected statistic does not appear in references
- Statistic field in TE takes on the edited name from Name field.
- copy -> paste as html is broken
- match table editor renaming action to table file destination
- TE > Batch to Figure does not work with Table with Keywords or Formulas
- TE batch to figure should play Success sound like the other batch functions
- Batched Table with Expected Range Hides the currently selected row
- TE Formula name dropped replaced with pre-current stat name in edit mode
- Batched Table is cutoff after selecting Resize Columns.
- Dont Show Again checkbox does not work from Batched Table to Add to Layout.
- Line Plot near 0 ticks missing
- Templates
- Template with Iteration set to Sample, Panel or keyword throws stack
- changes to log scale or miltenyi transform dont save to template
- on template generated workspace, changing graph type in LE looses population of graph
- First Time Opening Template with LE chart, chart is empty
- Group-applied gating not being applied with gating on comped parameters in templates
- Error Opening Template w/’Use Gating-ML Conventions’ Pref Enabled
- Error Opening Template w/’Show Ancestry’ Pref Enabled
- Text/Stat Box
- When adding FJML keywords, can’t separate then with return characters
- Fontsize in LE dropdown not adjusting correctly, displays wrong font size
- Figure Presets can’t add enter new name
- Text boxes are in edit mode when drawn but still can’t type text
- Transforms
- Miltenyi Template loses transform from wsp to wspt
- Graph in axis transform dialog not fully reset after cancel
- Manual transformation adjustment should be disallowed for default arcsinh tranform of CyTOF data
- Transform Window can draw range gates on graph
- Transform Editor – Next/Previous Sample Buttons Broken
- Y axis is missing the zero when showing transformed data
- UI Beautification
- slider starts at end point of scroll
- All Samples group not locked at top
- ToolTips do not dismiss when another app gains focus.
- Undefined
- engine requests when resizing window
- Staggered and Offset Histograms do not label X Axis on Overlays
- Influx data is causing analysis to freeze
- Background of gate annotation doesn’t change with fill
- contours look different in LE than GW
- Secondary graph windows don’t update when gate deleted
- PageUp and PageDown don’t work in inspect properties dialog
- overlay fill (via legend) menu shows “40%” option twice
- performance exponentially worsens as curly quads moved
- Editing keywords uses font too big for the space
- Create a Series of Values window transition
- can’t adjust gates when range is small
- ACS File Opens a Blank Workspace
- Zip containing fcs files not loaded
- column values are lost
- batch to web clips charts
- Gates on derived parameter fails in reopened workspace
- Arcsinh Transform Edits not Restored on Workspace reload
- Axes names not good for partec files
- Undo/Redo
- Undo does not change the Graph type back to original.
- Redo does all 3 overlays and skips the 2nd action overlay in the LE.
- Font size/style in Fonts dialog box does not reflect Undo update and get stack
- Undoing a staggered offset overlay in the LE is causing graph to show no pop
- Undo removes 2 separate Group apply’s and the Grandchild node in the WS changes to Sample level.
- Clicking Undo on an Overlay in the LE still shows the plots in the graph.
- Undo not implemented on Transform changes
- Undo delete legend in LE does not return legend
- Samples added should not be undoable when part of a wsp load
- All Quad gate movements aren’t Undone using Undo, the gates are just removed.
- Workspace
- Stats with no params do not update value when added to group
- Group applying 2 levels deep gates does not group apply the 2nd level gate and throws a stack.
- Stack is thrown when clicking on a group.
- Stack is thrown dragging and dropping a sample on another sample.
- Workspace thinks its connected to sample but is not.
- copy paste from excel does not work
- Lists disappear when scrolled – W7 java7 only
- Repositioning workspace column is causing column data to be duplicated
- Making a group with a staining selection shows up empty upon completion.
- There is a (-) replacing the Reagent Keywords in the Group dialog.
- Cannot add a gate onto a parent of the same name
- Quitting FlowJo with unsaved workspace asks twice to save the workspace when cancelling
- group inclusion is live but gate inheritance is not
- Save as ACS does not open finder
- Cant rename group via right click menu (sometimes)
- Right Click in Group Window is ignored if Group Col. is not leftmost
- FJ workspace icon tool tip seems to be outdated
- Loading Samples via Drag and Drop Adds to All groups
- FJ workspace icon Print functions are missing.
- group-inclusion-triggered inheritance of gates can yield frequencies greater than 100%
- Freq. of… quick stats
- Demo Mode warns about non demo files but DOES add them to the workspace. (They have no data)
- beta notification was not working
- Copy/Paste gates in WS doesn’t copy correct hierarchy if different node
- Dragging samples from desktop to group does not add to manual group
- Dragging samples from desktop to Group Analysis switches to All Samples
- Edit Group Properties shows a stringname.
- Keyword Value Series… Dialog does not update Keyword entry boxes when updating params below
- Event # parameter not copying to Group
- Reopen Graph Windows … not working right, remove for 10.0.7
- Creating NOT gate the stat of the original gate is lost
- Dragging gates between workspaces creates child gate rather than replacing same-named gate
- Identical Gate sample is using wrong icon
- Change “Autoquads” to “BifurGate”
- Cut, Copy, Paste of fcs files does not work
- Tooltip shows stringname and should be greyed out on 2nd level etc.
- Non-native group copied nodes are retained in WS but do not update when first shown or calculate without forcing
- Help icon in workspace doesn’t have tooltip
- Selection in the workspace with shift key only works when shift clicking on an object ABOVE the selection
- Dragging gates from workspace onto another one replaces existing Gate, but shows as group owned still
- No warning when opening lots of Inspect windows
- Grouped tethered gates do not delete from group
- statistic and cell count in the workspace are wrong/missing
- Groups cannot be collapsed in Group pane
- too many gates dropped from one wsp to another
- Comp Badge shows red on re-opened workspace
- Can’t drag samples, gates, or stats between workspaces
- Disclosure Triangle Hit detection fails as you move many levels deep
- tethered gates not applying correctly to group
- Disclosure Triangle does not work when not the leftmost column
New Features:
- Cell Cycle (New Platform)
- Export
- CSV Export added parameter annotation
- Export dialog defaults the output format to FCS3
- Exporting compensated parameters into new FCS files (new checkbox)
- Plates
- Added a heat map setting for an entire table, not just per column for Plates
- NEW: Plate drawing tool for the Layout Editor
- Layout Iteration: Removed the white box around the up and down buttons from the Iteration Value.
- Layout Plates: Added Legend for Plate Editor Heatmaps
- Look & Feel: Fixed Text color tone on FJ STart up icon (was too dark).
- Plate Editor (New Platform)
- Plate Editor Moved Both Icons from the Export Ribbon to the Document Ribbon, remove the Export Ribbon
- Drag and drop set of keywords from Staging well to individual wells, rows, and columns
- Ability to export Plate editor annotation as a .csv or .xls file
- Additional Selection capability in Plate Editor
- Plate Editor Create group button
- Added selecting wells in plate editor from group
- Double clicking on a value cell will make it editable.
- Right Click options on a wells
- Put name groups under staging wells
- Add a Load Samples from Group button
- Well Highlight for Keyword/Value pair feature change
- Add labels to lists on Plate Editor
- Plate Editor Well Copy Paste works when filtered on Keyword.
- Preferences
- Prefs for printing in the Layout Editor and rulers to update to the prefs set
- Add preferences for Default Histogram Style.
- Feature: Accuri data FSC & SSC should have different defaults
- Default scaling for Sony SP6800 data
- Don’t Show Again Prefs – reset now, reset on exit session. Also made them more granular, each dialog has it’s own setting.
- Added Plate Prefs
- Mirrored Layout Prefs & options in Layout Editor batch band
- Cytometers preferences scrollbar added.
- Ribbons: added ability to get to help on a ribbon when it is collapsed
- Table Editor
- Moved Keyword icon from Format/Keywords band to Edit/Columns band
- TE batched report Menu Re-design
- TE Batched Report Menus: added a “Hide/Show Summary Stats ” menu item
- Transforms: Change default scaling for Cytof data d/t data compression at zero
- Workspace
- added ability for keywords to move to the top of the list once used
- Clicking on a compensation node opens comp editor
- Change name of “Instrumentation” band to “Cytometry” (workspace tab)
- File Missing Icon is now easier to see
Known issues:
Flowjo 10 0 Vs
- 3D Viewer
- Axis are malformed when opened on comp parameters
- 3D viewer does not update live with gate moves in the GW
- Group applying a 3D node changes the parameters and the default color of the 3D plot.
- terrain plot monochromatic, missing 3rd axis label.
- Points Plotted at 0% on TE 3D Plot
- Annotation: Annotate Exp. Figures Tab: Preview of the fig should be scaled to fit
- Boolean Gates
- Combinations of NOT Gates with AND or OR gates has problems
- Identical Gates are not Clone gates
- Multidimensional Cell Cycle is still under construction.
- Compensation Matrix Editor
- Cannot undo Matrix application
- Group-applied size gates in compensation persist after deletion after compensation has been calculated
- Compensation
- Round assignment buttons do not accommodate long sample names
- Setting the wrong size gate with some Accuri data with AutoComp (Manual gate works fine)
- Strange scatter gates for S3 comp files
- S3 comp gate snaps to original position after unrelated axis transform
- ACS: No progress indication when opening ACS file
- Export: PDF export error when legend breaks pages
- File Formats
- Non-ascii char in fcs file may be stripped from file display in workspace
- 9.7.1 jo files: Bisector gate is converted to two range gates.
- 9.7.1 jo files: Quad gates converted to 4 rect gates.
- Graph Window
- “Save Graphic” as SVG doesn’t produce re-scalable vector object
- When pasting from GW to LE, Median (Black Cross) is gone
- Changing Oval Gate midpoint is reset when changing it’s endpoint
- Can’t select/delete quad gates in Graph window if other gates coexist.
- Kinetics is under construction
- Launch: Java network connections dialog flashes at startup after
- Layout Charts: Cannot turn legend ON and add keywords at the same time.
- Layout Editor
- Batching NxN too many times FJ will run out of memory or throw a stack.
- Batch to PPT w/tint, tint is solid
- Layout presets do not change anything but object settings on figures (as designed)
- Avoid Page Breaks – annotations moved incorrectly (or not moved at all) sometimes
- unable to paste image with transparency into the le
- Batched tables in LE do NOT live update
- Show gate median not appearing in LE
- graph bleed-thru in the LE when changing layers of overlapping graphs
- Dragged oval into grid object does not appear until grid resize
- batch to PDF ignores page orientation
- Copied Text Box retains wrong font size info
- layout editor window is collapsed upon editor opening (for some)
- FJML Blocks overlap slightly after copy and paste Text object.
- Layout Outputs: Clipping bug – SVG displaying gate outside graph area
- Layout Overlays
- Multi Graph Overlays size – lost when batching (reset to default size.)
- Renaming populations can break overlays / legends in the LE
- Panel Wizard: Panel Wizard has problems when it loses network connection.
- Population Comparison: Drag Compare Population Node to LE has lots of errors in console, most of chart is has no values
- Preferences: duplicate Cytometer preferences not being resolved
- Printing: The LE shows the data from the GW Print Dialog and disregards anything else currently in the LE.
- Shortcut Keys: When closing a GW through shortcut key Ctrl+W it’s also closing the WS (this is due to the key auto-repeat firing off the same events, this can be adjusted in the preferences, Diagnostics->Action Delay)
- Statistics: adding stats to wide samples is very slow and can crash FJ (Example file had 83 parameters)
- Table Editor
- TE resizing columns leaves unused space on right side of window
- TE: Formatting band does not work outside of the Visualize Tab
- Hide Column Checkbox removed from Edit Column Dialog
- Batched Table XML Excel does not show Column headers as seen on a regular Batched Excel
- table column headers change when saved as .wsp then opened in new workspace when to column title provided
- Text/Stat Box: copy paste in LE text box not working well
- Transforms
- Calculating indicator is missing when transform are applied
- S3 GW and Transform window parameters do not match up
- Platform Specific…
- We do not support Java 7. Windows versions include Java 6, and Java 6 can be installed with OSx along with Java 7 so FlowJo can use Java 6
- Retina displays not supported at high resolution.
- Directories with identically named data (ie plates, panels) confuse FJ grouping
- SampleID scale incorrect – concatenated files
- Derived Parameter window cancel doesn’t cancel… it applies.
- Creating a Formula with a Control Population withut supplying a name for the column may generate incorrect stats.
- We canot handle exporting separate files from barcode files with multiple populations in them.
- Undo/Redo
- Note: Undo and Redo may have unexpected results. We hope this is better in a future release. If you have specific cases where undo or redo is failing, please let us know.
- Undo Removes extra gate in WS
- Undo Brings back a deleted gate from unrelated operation
- Workspace
- Insufficient progress when opening large WSP files…
- User not provided feedback that workspace is still calculating on long calculations
- Double clicking on left side of group in Group pane does not open group. (Click further right)
- Cmd – W (Alt -W) Should close the frontmost window (and does not always close it)
- Spacing in the rows and columns of the WS changes when Preference dialog is opened then closed.
- Gate annotations don’t update w/synchronized option (but the gates do!)